Studies on sperm capacitation. V. Characterization of decapacitation factor from guinea-pig spermatozoa.
نویسندگان
چکیده
منابع مشابه
Characterization of receptors for sulphated polysaccharides on guinea-pig spermatozoa.
give 50"h inhibition of binding ( lC5,,) was derived from these graphs. The values for ram proacrosin were as follows: unlabelled fucoidan ( 19.1 nM), dextran sulphate SO0 000 M, (3 1.6 nM), dextran sulphate 5000 M, (251 nM), polyvinylsulphate (326 nM), xylan (299 p ~ ) and mannan ( 1000 p ~ ) . The following solutions were tested. but did not reach 50%) inhibition at the concentrations given: ...
متن کاملEffects of Ca and HCO3 on Capacitation, Hyperactivation and Protein Tyrosine Phosphorylation in Guinea Pig Spermatozoa
In our previous report, we demonstrated that the tyrosine phosphorylation of sperm proteins (TPSP) of guinea pig was associated with capacitation and hyperactivation (CAHA), and Ca and HCO3 were required for the initiation of CAHA and increasing the TPSP. The aim of this study was to further investigate the mechanism underlying the above events. The results showed that addition of cAMP agonists...
متن کاملLeptin and leptin receptor in pig spermatozoa: evidence of their involvement in sperm capacitation and survival.
Several studies have recently investigated the role of leptin, the adipocyte-secreted hormone, in the growth and reproduction of rodents, humans, and domestic animals. The present study was designed to explore the expression of leptin and its receptor in pig spermatozoa. Successful Western blot evidenced a 16 kDa band for leptin and six isoforms, ranging from 120 to 40 kDa, for the leptin recep...
متن کاملCapacitation-associated Changes in Protein-tyrosine-phosphorylation, Hyperactivation and Acrosome Reaction in Guinea Pig Sperm*
The aim of this study was to evaluate the effects of Ca, HCO3 and BSA on the in vitro capacitation-associated protein tyrosine phosphorylation, hyperactivation and acrosome reaction in guinea pig sperm. Caudal epididymal sperm were incubated in four different groups: modified TALP (Tyrode’s albumin lactate pyruvate) or TALP without one of the medium constituents (Ca, HCO3 and BSA). After incuba...
متن کاملMembrane potential of guinea-pig spermatozoa.
The membrane potential of guinea-pig spermatozoa was investigated by using the fluorescent dye, diS-C3-(5). The potential did not vary when K replaced Na in the suspending medium, and in a physiological salt solution the resting potential was found to be about +13 mV.
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1976
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.24.907